Phloretin Powder

1. Raw Material Screening and Pretreatment: Focusing on High-Content Natural Sources

Kintainutri® prioritizes natural, high-content raw materials. Its core raw materials come from the root bark and unripe peels of apple trees (Malus domestica). We also supplement with discarded peels and pomace from the apple processing industry (juice and jam production) to recycle resources and reduce production costs.

Prior to storage, raw materials undergo a triple screening process: ① Testing for variety purity (to ensure they are the target Rosaceae species); ② Eliminating moldy or impurity-prone raw materials; ③ High-Performance Liquid Chromatography (HPLC) testing for initial phloretin (and phlorizin) content. Only raw materials with a content of 1.2% or higher are selected for the next step. The raw materials are then cleaned, dried to a moisture content of ≤8%, and crushed to a 40-60 mesh size for subsequent extraction.

2. Extraction Stage: Combining Multiple Methods to Efficiently Release Active Ingredients

Enzymatic Pretreatment: Because phlorizin primarily exists in the form of a glycoside (phlorizin), Kintainutri® first utilizes enzymatic hydrolysis. Food-grade β-glucosidase is added to the crushed raw material. The reaction is then maintained at 35-40°C and a pH of 5.0-5.5 for 2-3 hours. This fully hydrolyzes the glycosidic bonds of phlorizin, releasing free phlorizin and improving subsequent extraction efficiency.

Supercritical CO₂ Extraction: Eliminating the residual risks associated with traditional organic solvent extraction, Kintainutri® utilizes industrial supercritical fluid extraction (SFE) technology. Using food-grade CO₂ as the extractant, the enzymatically hydrolyzed raw material undergoes dynamic extraction for 1.5-2 hours under a supercritical pressure of 30-35 MPa and a temperature of 40-45°C. Leveraging the high permeability and solubility of CO₂, the phloretin is efficiently separated from impurities. Following extraction, the CO₂ is vaporized and recovered (for recycling) by reducing the pressure and temperature to yield a crude phloretin extract. This process eliminates solvent residue and maximizes the preservation of phloretin's natural activity.​

3. Purification and Refining: A Step-by-Step Process Ensures High Purity

Column Chromatography Purification: The crude extract is passed through a chromatography column packed with macroporous adsorption resin. Gradient elution (using an ethanol-water solution as the eluent) is performed to separate phloretin from other polyphenol impurities. The target fractions are collected and concentrated using a rotary evaporator at 50-55°C to obtain a phloretin concentrate (with a concentration increased to 30%-40%).

Recrystallization Purification: An appropriate amount of deionized water is added to the concentrate. The concentrate is allowed to stand at 5-10°C for 8-10 hours to precipitate phloretin crystals. The crystals are then collected by centrifugation (3000-3500 rpm) and washed 2-3 times with purified water to further remove residual impurities, resulting in phloretin crystals with a purity of ≥95%.

 

4. Drying to Powder and Packaging: Balancing Activity and Practicality

Vacuum Freeze-Drying: To prevent high temperatures from destroying the activity of phloretin, Kintainutri® utilizes vacuum freeze-drying technology. Phloretin crystals are frozen to a solid state at temperatures below -40°C. Sublimation dehydration occurs under a vacuum environment (pressure ≤10 Pa), resulting in a loose, coarse phloretin powder.

This powder is then pulverized and passed through an 80-100 mesh sieve, ultimately yielding a white to pale yellow, uniformly sized phloretin powder with a consistently high purity of over 98%. Customized Packaging: After drying, the phloretin powder is immediately packaged in a cleanroom (Class 10,000 cleanliness level).

Kintainutri® offers customized packaging services based on customer needs: small-batch trial packs (100g/food-grade aluminum foil bag, sealed to prevent light), regular production packs (1kg/aluminum foil vacuum bag with desiccant), and large-volume purchase packs (25kg/pharmaceutical-grade HDPE drum lined with food-grade plastic bag). Neutral packaging or customized printing are also available to ensure product activity is maintained during storage and transportation.

5. Full Quality Inspection: Throughout the entire production process

From raw materials to finished product, Kintainutri® undergoes rigorous testing at every stage: Testing for heavy metals (lead, arsenic, and mercury) and pesticide residues at the raw material stage; testing for enzymatic hydrolysis efficiency and extraction yield during extraction; and HPLC testing for purity during purification. In the finished product stage, in addition to testing for purity, moisture, and particle size, microbiological indicators (total colony count, mold and yeast counts) are also verified to ensure compliance with ISO9001, ISO22000, and national standards.

Kintainutri® phloretin powder should be stored sealed in a cool, dry place (temperature ≤ 25°C, humidity ≤ 60%), away from direct sunlight and high temperatures to prevent degradation. Operators are advised to wear dust masks and gloves during the production process to reduce inhalation of powder or direct contact with skin. If it accidentally comes into contact with eyes, rinse immediately with plenty of water to ensure safe operation.